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Vitamin B12 is absorbed by the body during the digestive process and requires a protein that is made by the stomach called the intrinsic factor

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NMR spectroscopy Samples for NMR measurements contained 300 μM 15N labeled FOXO4 86–206 protein in 50 mM sodium phosphate, pH 6.5, 150 mM NaCl and 1 mM DTT with 10% 2H 2 O added for the lock signal